The Freezing of Plant Tissue

Windchill and the risk of tissue freezing.

Low air temperatures and high wind speeds are associated with an increased risk of freezing of the exposed skin. P. A. Siple and C. F. Passel (Proc. Am. Phil. Soc. 89: 177-199, 1945) derived their windchill index from cooling experiments on a water-filled cylinder to quantify the risk of frostbite. Their results are reexamined here. It is found that their windchill index does not correctly desc...

Snap-freezing of Post-surgical Tissue Biospecimens

This evidence-based best practice document is applicable to all human tissues that are to be preserved by snap-freezing. Biospecimens preserved under these procedural guidelines are suitable for downstream analysis of DNA, RNA, protein, and morphology endpoints. Additional analytical endpoints, including but not limited to cell viability, cell sorting, drug sensitivity testing, or use as donor ...

Snap-freezing of Post-surgical Tissue Biospecimens

This evidence-based best practice document is applicable to all human tissues that are to be preserved by snap-freezing. Biospecimens preserved under these procedural guidelines are suitable for downstream analysis of DNA, RNA, protein, and morphology endpoints. Additional analytical endpoints, including but not limited to cell viability, cell sorting, drug sensitivity testing, or use as donor ...

Osmotic shrinkage as a factor in freezing injury in plant tissue cultures.

Haplopappus gracilis and Acer saccharum tissue culture cells are extremely sensitive to freezing injury, and exhibit a decrease in survival from 98% at -1 C to 4% at -3 C (Haplopappus) and 92% at -3 C to 13% at -5 C (Acer) when suspended in distilled H(2)O, seeded at -1 C, and then cooled by 0.1 C/minute. Similar results are obtained when cells are suspended in growth medium. The extent of shri...

The Starch Isolated from Plant Material by the Freezing Method